Mycobacterium phage Phussie
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| Detailed Information for Phage Phussie | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Ava Smith |
| Year Found | 2022 |
| Location Found | Collegeville, PA United States of America |
| Finding Institution | Saint Joseph's University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.141692 N, 75.474 W Map |
| Discovery Notes | Found in Providence View Neighborhood Park in Collegeville, Pennsylvania. The soil was near a pond and next to a reed swamp. |
| Naming Notes | When trying to isolate this phage, I encountered many difficulties. I often had to redo purification rounds and Lysates in order for the phage to appear on the plate. Because of this, I referred to my phage as "fussy". A classmate and I used to joke and put the prefix "ph-" in front of everything phage related. Therefore, phussie. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques appeared very small (0.9 mm) and clear. The plaques emerged after 48 hours in the incubator. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 146 |
| Pitt Freezer Box Grid# | B9 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |