Streptomyces phage Pondspawn
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| Detailed Information for Phage Pondspawn | |
| Discovery Information | |
| Isolation Host | Streptomyces mirabilis NRRL B-2400 |
| Found By | Zainab Bharmal & Meghan Kwon |
| Year Found | 2022 |
| Location Found | Baltimore, MD United States |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.258492 N, 76.596541 W Map |
| Discovery Notes | We obtained our bacteriophage from a soil sample taken from UMBC Library Pond. The sample was collected on 9/14/2022 at a temperature of 75 degrees. We performed direct isolation on 9/15/2022. We incubated overnight and performed our first round of phage purification through plaque assay on 9/22/2022. |
| Naming Notes | We decided to name our phage: Pondspawn as we found our phage at the UMBC pond! |
| Sequencing Information | |
| Sequencing Complete? | No |
| Sequencing Facility | University of Pittsburgh Genomics and Proteomics Core Laboratories |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | BH |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | After conducting the lysogeny test, we found that our phage is not a lysogenic phage. After incubation of 48 hours, we did not see any evidence of bacteria on the plates. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques from our phage are turbid and cloudy. These plaques are round and circular, and they are about 2 mm diameter. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 151 |
| Pitt Freezer Box Grid# | D8 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |