Mycobacterium phage Porklet
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| Detailed Information for Phage Porklet | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Julian Ramprashad |
| Year Found | 2021 |
| Location Found | Providence, RI United States |
| Finding Institution | Brown University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 70°C |
| GPS Coordinates | 41.817606 N, 71.391772 W Map |
| Discovery Notes | This sample was taken from the underside of a grassy ridge located a few feet above the riverbank of India Point Park in Providence, RI. The soil was taken approximately an inch deep into the side of the ridge, which was about 5-7 inches below the grassy upper surface. The soil was dry, fine, and relatively loose. Plaques were observed after sample enrichment. |
| Naming Notes | Although most restriction enzyme and PCR results suggest that this phage is in subcluster B1, some other results, such as the Xho1 and Bsu15I restriction enzyme cutting patterns, were inconsistent with previously-documented B1 phage. The name “Porklet” is a variation on “Hamlet,” which is a reference to the play containing the famous phrase “to [B1] or not to [B1].” |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaques have appeared as either completely clear or as having a clear center surrounded by a partially-cloudy ring. Plaque diameter varies but is often within the range of 3-5 mm. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
