Mycobacterium phage Problematic
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Detailed Information for Phage Problematic
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMiranda Raevsky
Year Found2012
Location FoundCanton, MO USA
Finding InstitutionCulver-Stockton College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates40.128635 N, 91.530101 W Map
Discovery NotesThe sample the phage was collected from was gathered on 9/6/2012 at approximately 9:20 a.m. on the Culver-Stockton College campus. The outside temperature was 85 degrees Fahrenheit. The sample was collected approximately 4 centimeters below the surface from a moist soil close to a tree.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe plaques for this phage went through cycles of changing sizes even after purification. The plaques were initially fairly large but after about two weeks they began to appear as small plaques. The cycle went back and forth between big and small approximately every two weeks. The plaques always appeared to be lytic phages.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 13.4 x 10^10 pfu/mL
Date of SEA Lysate Titering Nov 5, 2012
Pitt Freezer Box# 43
Pitt Freezer Box Grid# 24
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload