Mycobacterium phage Quinoa
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| Detailed Information for Phage Quinoa | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Madison Feria |
| Year Found | 2020 |
| Location Found | Bowling Green, KY United States of America |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 36.950051 N, 86.434167 W Map |
| Discovery Notes | I was unable to attend the first two weeks of lab due to COVID exposure. I began working with my phage during serial dilutions. I did have odd results for a lot of my dilutions as plates with a higher dilution produced more plaques that plates of a lower dilution. When I first flooded a web plate, my titer was below the recommended 10^9. After preparing a ten-plate lysate, my phage concentration increased to 1.37x10^10. |
| Naming Notes | Quinoa is one of my favorite foods, and I happened to be eating it while I was working on my notebook for this lab. I also chose quinoa because my plates sometimes looked like a plate full of quinoa. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques were almost completely clear. Only a few plaques on each plate would appear slightly turbid. The plaques ranged in size between 0.2 and 0.5 cm with the average being around 0.3 cm. My plaques did not change over the course of this semester. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.37 x 10^10 |
| Pitt Freezer Box# | 120 |
| Pitt Freezer Box Grid# | F6 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
