Mycobacterium phage Raphael
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Detailed Information for Phage Raphael
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMartha Dua-Awereh
Year Found2010
Location FoundBrooklyn, NY USA
Finding InstitutionBrooklyn College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates40.60174 N, 73.93079 W Map
Discovery NotesPhage was located in a water/soil mixture taken 8 inches from the shoreline of the the Marine Park Salt Marsh, in shallow water and about 10 feet from phragmites reeds.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesRaphael is a lytic phage that forms clear plaques in lawns of M. smegmatis. The serial dilutions performed indicate that the phage forms 3.02 x 10e12 plaque-forming units per mL of phage isolate solution (phage in 7H9 complete media). Each plaque is 0.2 cm wide in diameter.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2010BROOraphaelMD
SEA Lysate Titer 3.0e12
Date of SEA Lysate Titering Aug 1, 2010
Pitt Freezer Box# 11
Pitt Freezer Box Grid# 39
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload