Microbacterium phage Rasputia
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Detailed Information for Phage Rasputia
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Found ByJelsia Cottone and Gabrielle Berwick
Year Found2019
Location FoundLanghorne, PA USA
Finding InstitutionUniversity of the Sciences in Philadelphia
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature28°C
GPS Coordinates40.160561 N, 75.002129 W Map
Discovery NotesFound in a garden containing moist dark brown colored soil. Close to homes and main roads. With lots of foot trafficking done by a cute dog.
Naming NotesThe name of this phage is Rasputia. It came from a movie that we believe fit the characteristics of our phage when we saw the EM screening.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 20, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage47
Genome length (bp)97105
Character of genome endsCircularly Permuted
GC Content66.3%
Sequencing NotesThe phage was sequenced as part of a DOGEMS sample.
Fasta file available?Yes: Download fasta file
Characterization
ClusterGC
Subcluster--
Cluster Life CycleUnknown
Lysogeny NotesHigh lysate titer was calculated to 8.3 X 10^8 pfu/mL.
Other Cluster Members

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Annotating InstitutionUniversity of the Sciences in Philadelphia
Annotation StatusBeing Annotated (Expected completion by 5/1/2020)
Plaque NotesIt appears to be a lytic life cycle, because the plaques edges are small, clear and circular. The plaque sizes are 1.42 mm.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 8.3 x 10^8
Available Files
Plaque PictureDownload
EM PictureDownload