Microbacterium phage Rasputia
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Detailed Information for Phage Rasputia
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByJelsia Cottone and Gabrielle Berwick
Year Found2019
Location FoundLanghorne, PA USA
Finding InstitutionUniversity of the Sciences in Philadelphia
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature28°C
GPS Coordinates40.160561 N, 75.002129 W Map
Discovery NotesFound in a garden containing moist dark brown colored soil. Close to homes and main roads. With lots of foot trafficking done by a cute dog.
Naming NotesThe name of this phage is Rasputia. It came from a movie that we believe fit the characteristics of our phage when we saw the EM screening.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 20, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage47
Genome length (bp)97105
Character of genome endsCircularly Permuted
GC Content66.3%
Sequencing NotesThe phage was sequenced as part of a DOGEMS sample.
Fasta file available?Yes: Download fasta file
Characterization
ClusterGC
Subcluster--
Cluster Life CycleUnknown
Lysogeny NotesHigh lysate titer was calculated to 8.3 X 10^8 pfu/mL.
Other Cluster Members

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Annotating InstitutionUniversity of the Sciences in Philadelphia
Annotation StatusIn GenBank
Plaque NotesIt appears to be a lytic life cycle, because the plaques edges are small, clear and circular. The plaque sizes are 1.42 mm.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Rasputia_1
(ParB-like nuclease domain)

Rasputia_2
(ParB-like nuclease domain)

Rasputia_3
(ParB-like nuclease domain)

Rasputia_4
(helix-turn-helix DNA binding domain)

Rasputia_5
(terminase)

Rasputia_6

Rasputia_7

Rasputia_8

Rasputia_9

Rasputia_10
(DNA ligase)

Rasputia_11

Rasputia_12

Rasputia_13

Rasputia_14

Rasputia_15

Rasputia_16

Rasputia_17

Rasputia_18

Rasputia_19

Rasputia_20

Rasputia_21

Rasputia_22

Rasputia_23

Rasputia_24

Rasputia_25
(DnaQ-like (DNA polymerase III subunit))

Rasputia_26

Rasputia_27

Rasputia_28

Rasputia_29

Rasputia_30

Rasputia_31

Rasputia_32

Rasputia_33

Rasputia_34

Rasputia_35

Rasputia_36
(HNH endonuclease)

Rasputia_37

Rasputia_38

Rasputia_39

Rasputia_40

Rasputia_41

Rasputia_42

Rasputia_43

Rasputia_44

Rasputia_45
(portal protein)

Rasputia_46

Rasputia_47

Rasputia_48

Rasputia_49

Rasputia_50

Rasputia_51

Rasputia_52
(Mu-F like minor capsid protein)

Rasputia_53

Rasputia_54
(capsid maturation protease)

Rasputia_55

Rasputia_56
(major capsid protein)

Rasputia_57

Rasputia_58

Rasputia_59
(major tail protein)

Rasputia_60

Rasputia_61
(phosphoesterase)

Rasputia_62
(head-to-tail adaptor)

Rasputia_63

Rasputia_64

Rasputia_65

Rasputia_66

Rasputia_67

Rasputia_68

Rasputia_69
(tail assembly chaperone)

Rasputia_70
(tail assembly chaperone)

Rasputia_71
(tape measure protein)

Rasputia_72
(minor tail protein)

Rasputia_73
(minor tail protein)

Rasputia_74
(minor tail protein)

Rasputia_75

Rasputia_76

Rasputia_77

Rasputia_78
(lysin A)

Rasputia_79

Rasputia_80

Rasputia_81

Rasputia_82

Rasputia_83

Rasputia_84

Rasputia_85

Rasputia_86

Rasputia_87

Rasputia_88

Rasputia_89
(DNA polymerase I)

Rasputia_90

Rasputia_91

Rasputia_92
(DNA primase/polymerase/helicase)

Rasputia_93

Rasputia_94

Rasputia_95

Rasputia_96

Rasputia_97

Rasputia_98

Rasputia_99

Rasputia_100

Rasputia_101

Rasputia_102

Rasputia_103

Rasputia_104

Rasputia_105

Rasputia_106

Rasputia_107

Rasputia_108

Rasputia_109

Rasputia_110

Rasputia_111
(DNA helicase)

Rasputia_112

Rasputia_113

Rasputia_114

Rasputia_115
(Cas4 family exonuclease)

Rasputia_116

Rasputia_117

Rasputia_118

Rasputia_119

Rasputia_120

Rasputia_121

Rasputia_122

Rasputia_123

Rasputia_124

Rasputia_125

Rasputia_126
(rtcB-like RNA ligase)

Rasputia_127

Rasputia_128

Rasputia_129

Rasputia_130

Rasputia_131
(MazG-like nucleotide pyrophosphohydrolase)

Rasputia_132

Rasputia_133

Rasputia_134

Rasputia_135

Rasputia_136
(AAA-ATPase)

Rasputia_137

Rasputia_138
(nuclease)

Rasputia_139

Rasputia_140

Rasputia_141

Rasputia_142

Rasputia_143

Rasputia_144

Rasputia_145

Rasputia_149

Rasputia_150

Rasputia_151
(glycosyltransferase)

Rasputia_152
(glycosyltransferase)

Rasputia_153
(glycosyltransferase)

Rasputia_154
(acetyltransferase)

Rasputia_155
(methyltransferase)

Rasputia_156
(PAPS reductase-like domain)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMT639646
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 8.3 x 10^8
Pitt Freezer Box# 98
Pitt Freezer Box Grid# D10
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload