Mycobacterium phage Saboteur
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Detailed Information for Phage Saboteur
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Former namesDebbieJS
Found ByCristina Scanlon
Year Found2021
Location FoundPhiladelphia, PA United States
Finding InstitutionUniversity of the Sciences in Philadelphia
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature5°C
GPS Coordinates39.945676 N, 75.208051 W Map
Discovery NotesThis sample was taken from the quad at University of the Sciences on February 16th, 2021 at 7:55AM, very close to the parking lot. The soil was collected from a 4 cm depth and was determined to be moist. This is an urban area. It was from a planter, so there is no foot traffic on the soil itself. The ground was not sloped and there were various grass plants in the planter. There was snow around the location as well.
Naming NotesThe soil sample originally went by as DebbieJS. Upon further discussion, our group thought Saboteur was a more appropriate name considering it means to deliberately damage or destroy something, much like the bacteriophage destroys the host bacteria.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe plaques present on the plaque assays present a clean cut clearing ranging between 1 mm to 2 mm in size, meaning that the bacteriophage has a lytic cycle.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 4.77E9 pfu/mL
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload