Mycobacterium phage Saboteur
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| Detailed Information for Phage Saboteur | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | DebbieJS |
| Found By | Christina Scanlon |
| Year Found | 2021 |
| Location Found | Philadelphia, PA United States |
| Finding Institution | University of the Sciences (now St. Joseph's Univ) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 5°C |
| GPS Coordinates | 39.945676 N, 75.208051 W Map |
| Discovery Notes | This sample was taken from the quad at University of the Sciences on February 16th, 2021 at 7:55AM, very close to the parking lot. The soil was collected from a 4 cm depth and was determined to be moist. This is an urban area. It was from a planter, so there is no foot traffic on the soil itself. The ground was not sloped and there were various grass plants in the planter. There was snow around the location as well. |
| Naming Notes | The soil sample originally went by as DebbieJS. Upon further discussion, our group thought Saboteur was a more appropriate name considering it means to deliberately damage or destroy something, much like the bacteriophage destroys the host bacteria. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques present on the plaque assays present a clean cut clearing ranging between 1 mm to 2 mm in size, meaning that the bacteriophage has a lytic cycle. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 4.77E9 pfu/mL |
| Pitt Freezer Box# | 130 |
| Pitt Freezer Box Grid# | C7 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
