Arthrobacter phage Saphira
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Detailed Information for Phage Saphira
Discovery Information
Isolation HostArthrobacter sp. ATCC 21022
Found BySarah Heady
Year Found2015
Location FoundWaco, TX USA
Finding InstitutionBaylor University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature38°C
GPS Coordinates31.547986 N, 97.120278 W Map
Discovery NotesDry soil was collected from a location under a tree next to an Exxon gas station near the campus of Baylor University in Waco, TX by Rozi H. The soil was enriched by Mackenzie Kallemeyn in fall 2014 and she archive DaBears. EeyoreJr was also isolated from this soil lysate.
Naming NotesUnder Mackenzie Kallemeyn, the phage was named "DaBears," but was never cataloged. Sarah Heady renamed the phage "Saphira" after the dragon in Eragon.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 28, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1348
Genome length (bp)15549
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCCCGCGCCACC
GC Content60.0%
Fasta file available?Yes: Download fasta file
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionNeumann University
Annotation StatusIn GenBank
Plaque NotesThe plaques produced by Saphira are consistently very small, smooth, and round, with an average diameter of 1-2mm.
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN617842
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.5*10^8 PFU/mL
Date of SEA Lysate Titering Nov 24, 2015
Pitt Freezer Box# 21
Pitt Freezer Box Grid# D11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload