| Detailed Information for Phage Sienna | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Bethany Hileman |
| Year Found | 2019 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.439538 N, 79.952256 W Map |
| Discovery Notes | The soil sample was collected on 09/09/2019 at 17:50 along the side of the house at 399 Argyle Way, Pittsburgh, PA, 15213. The ground where the sample was collected was covered with grass, but a shovel was used to collect soil from at or below 1 inch from the surface. The soil collected was moist and appeared dark brown, almost black. The weather at the time of collection was overcast and it began raining as the sample was collected, adding to the moisture of the sample. The soil sample was then stored on top of a desk at room temperature for approximately 19 hours and 10 minutes with minimal light exposure. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 8, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 4073 |
| Genome length (bp) | 66807 |
| Character of genome ends | Circularly Permuted |
| GC Content | 59.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | DG |
| Subcluster | DG1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | After at least 3 days of incubation at 30°C: range of small, circular plaques approximately 0.5-1.0mm in diameter with clear centers surrounded by turbid halos. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewSienna_1 Sienna_2 Sienna_3 Sienna_4 Sienna_5 Sienna_6 Sienna_7 Sienna_8 Sienna_9 Sienna_10 Sienna_11 Sienna_12 Sienna_13 Sienna_14 Sienna_15 Sienna_16 Sienna_17 Sienna_18 Sienna_19 Sienna_20 Sienna_21 Sienna_22 Sienna_23 Sienna_24 Sienna_25 Sienna_26 Sienna_27 Sienna_28 Sienna_29 Sienna_30 Sienna_31 Sienna_32 Sienna_33 Sienna_34 Sienna_35 Sienna_36 Sienna_37 Sienna_38 Sienna_39 Sienna_40 Sienna_41 Sienna_42 Sienna_43 Sienna_44 Sienna_45 Sienna_46 Sienna_47 Sienna_48 Sienna_49 Sienna_50 Sienna_51 Sienna_52 Sienna_53 Sienna_54 Sienna_55 Sienna_56 Sienna_57 Sienna_58 Sienna_59 Sienna_60 Sienna_61 Sienna_62 Sienna_63 Sienna_64 Sienna_65 Sienna_66 Sienna_67 Sienna_68 Sienna_69 Sienna_70 Sienna_71 Sienna_72 Sienna_73 Sienna_74 Sienna_75 Sienna_76 Sienna_77 Sienna_78 Sienna_79 Sienna_80 Sienna_81 Sienna_82 Sienna_83 Sienna_84 Sienna_85 Sienna_86 Sienna_87 Sienna_88 Sienna_89 Sienna_90 Sienna_91 Sienna_92 Sienna_93 Sienna_94 Sienna_95 Sienna_96 Sienna_97 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MT771345 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.67*10^11 pfu/mL |
| Pitt Freezer Box# | 115 |
| Pitt Freezer Box Grid# | C11 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
