Detailed Information for Phage Sisko |
Discovery Information |
Isolation Host | Gordonia terrae 3612 |
Found By | Max Ungrey |
Year Found | 2022 |
Location Found | Tampa, FL United States |
Finding Institution | University of South Florida |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | No |
Isolation Temperature | 32°C |
GPS Coordinates | 28.061111 N, 82.421389 W Map |
Discovery Notes | The phage was found in a marshy area near Shriners Children's Hospital. I took soil from a grassy area on the water's edge which emitted a strong musky odor. The humidity was 75% and there was no recent precipitation. |
Naming Notes | The phage is named after Commander Benjamin Sisko from the TV show Star Trek: Deep Space Nine. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Nov 28, 2022 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 798 |
Genome length (bp) | 62197 |
Character of genome ends | Circularly Permuted |
GC Content | 69.9% |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | DR |
Subcluster | -- |
Cluster Life Cycle | Lytic |
Other Cluster Members |
|
Annotating Institution | University of South Florida |
Annotation Status | In GenBank |
Plaque Notes | Plaques range in size from pinprick size to a diameter of up to 2mm. The plaques are temperate, so they have cloudy edges. |
Morphotype | Siphoviridae |
Number of Genes | 84 |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | OQ938590 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
SEA Lysate Titer |
5.71x10^9 PFU/mL |
Pitt Freezer Box# |
149 |
Pitt Freezer Box Grid# |
F9 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |