Gordonia phage Sproutie
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Detailed Information for Phage Sproutie
Discovery Information
Isolation HostGordonia terrae 3612
Found ByDesiree Paige Rose
Year Found2017
Location FoundPittsburgh, PA United States of America
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates40.44222 N, 79.95611 W Map
Discovery NotesThe soil sample was collected entirely in a clean plastic bag at approximately 10:30 on Monday January 23, 2017 with a temperature of 46°F. The soil was collected from a flower pot, and the soil was very moist, full of organic matter, and collected approximately 2 inches below the surface.
Naming NotesI named this phage with the help of my mother by considering the location of its origin. The soil sample was collected from a flower pot area, and plants sprout out of the ground, so Sproutie did too.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMay 16, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1967
Genome length (bp)46221
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceTACCAGGGGGA
GC Content66.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ2
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesThe plaque formed was small,round, and clear in color.
Has been Phamerated?Yes
Gene List

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Sproutie_1
(terminase small subunit)

Sproutie_2
(terminase large subunit)

Sproutie_3
(portal protein)

Sproutie_4
(capsid maturation protease)

Sproutie_5
(scaffolding protein)

Sproutie_6
(major capsid protein)

Sproutie_7
(head-to-tail connector complex protein)

Sproutie_8

Sproutie_9

Sproutie_10

Sproutie_11

Sproutie_12
(major tail protein)

Sproutie_13
(tail assembly chaperone)

Sproutie_14
(tail assembly chaperone)

Sproutie_15
(tape measure protein)

Sproutie_16
(minor tail protein)

Sproutie_17
(minor tail protein)

Sproutie_18
(lysin A)

Sproutie_19

Sproutie_20

Sproutie_21
(membrane protein)

Sproutie_22
(membrane protein)

Sproutie_23

Sproutie_24
(lysin B)

Sproutie_25
(minor tail protein)

Sproutie_26

Sproutie_27

Sproutie_28
(minor tail protein)

Sproutie_29

Sproutie_30
(excise)

Sproutie_31
(membrane protein)

Sproutie_32

Sproutie_33

Sproutie_34

Sproutie_35

Sproutie_36

Sproutie_37
(integrase)

Sproutie_38

Sproutie_39

Sproutie_40
(helix-turn-helix DNA binding domain protein)

Sproutie_41

Sproutie_42

Sproutie_43

Sproutie_44
(immunity repressor)

Sproutie_45
(helix-turn-helix DNA binding domain protein)

Sproutie_46

Sproutie_47
(DNA polymerase III sliding clamp)

Sproutie_48
(DNA polymerase III sliding clamp)

Sproutie_49

Sproutie_50

Sproutie_51

Sproutie_52

Sproutie_53
(exonuclease)

Sproutie_54
(RecT-like ssDNA binding protein)

Sproutie_55

Sproutie_56

Sproutie_57

Sproutie_58

Sproutie_59

Sproutie_60

Sproutie_61
(DNA methylase)

Sproutie_62

Sproutie_63

Sproutie_64

Sproutie_65

Sproutie_66

Sproutie_67

Sproutie_68
(RusA-like resolvase)

Sproutie_69

Sproutie_70
(helix-turn-helix DNA binding domain protein)

Sproutie_71

Sproutie_72

Sproutie_73

Sproutie_74

Sproutie_75
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK279911
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 56
Pitt Freezer Box Grid# D11
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload