Gordonia phage Squancho
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| Detailed Information for Phage Squancho | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Anna Bonitatibus |
| Year Found | 2018 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.444878 N, 79.960843 E Map |
| Discovery Notes | The phage was found directly beside a sidewalk approximately twenty yards from Irvis Hall at the University of Pittsburgh. The sample was collected at 8:59 P.M. on September 4th, 2018 on a warm and humid night at 83 degrees Fahrenheit. The nearby surroundings were pretty limited, mostly consisting of the sidewalk and some grass, yet there were some trees located about ten yards or so away. The soil which the sample was taken from was very dry, light brown in color and powdery. The sample was put in the fridge six minutes after collection, where it stayed for approximately 35 hours until I took it to the lab for isolation. |
| Naming Notes | I wanted to name a future pet of mine Squncho (which is based on an internet meme), which would translate phonetically to "Squincho", but for slightly greater sense I changed the phage name to "Squancho". |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Squancho's plaques were very clear and circular which indicates a lytic phage, although a lack of lysogeny testing makes the true temperate or lytic classification unknown. The plaques range from being around 1mm-3mm in size. Regardless of three careful rounds of purification, the plaques always varied among two different sizes with the sizes often being 1 or 3mm. From this, I concluded that my particular phage tended to produce different sized plaques regardless of there being only one phage present. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 2.0x10^10 pfu |
| Pitt Freezer Box# | 91 |
| Pitt Freezer Box Grid# | C1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |