Mycobacterium phage TheSymbiote
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Detailed Information for Phage TheSymbiote
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByTrinity Umaña
Year Found2021
Location FoundWilliamsburg, VA United States
Finding InstitutionCollege of William & Mary
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS Coordinates40.741895 N, 73.989308 E Map
Discovery Notessoil: Near a bush, slightly moist, dark, black

soil plates: lots of yeast and fungi, terrible lawn, a few possible plaques
Naming NotesNamed after the character/species in the Marvel Venom comics because the bacterial goop and gunk looked like the symbiote when transferring the aqueous phase of the lysate mixture.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque Notesbehavior: very inconsistent, produced results that were the opposite of what we expected every time

DNA: produced a lot of DNA precipitate, plates did not line up with the amount of DNA extracted, plates either lysed or overgrown except for 10^-8 dilution which had abt 500 plaques.

gel electrophoresis: cut very cleanly in multiple spots
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 160
Pitt Freezer Box Grid# H8