Corynebacterium phage TouchMeNot
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Detailed Information for Phage TouchMeNot
Discovery Information
Isolation HostCorynebacterium vitaeruminis CAG1
Duplicates of this PhageCloverDale
Found ByKerry Iles
Year Found2017
Location FoundPittsburgh, PA USA
Finding InstitutionUniversity of Pittsburgh
ProgramPhage Hunters Integrating Research and Education
From enriched soil sample?No
Isolation TemperatureNot entered
GPS Coordinates40.4375 N, 79.9465 W Map
Discovery NotesThis phage was isolated from the plant Impatiens pallida, collected by Dr. Tia-Lynn Ashman. The plant was washed with PYCa, filtered, and then enriched with host at 30 degrees Celsius. Purification was performed by Kira Zack.
Naming NotesThis phage was isolates from Impatiens pallida, a plant from the Touch-Me-Not family, Balsaminaceae.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 6, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2171
Genome length (bp)40526
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCACCCAT
End Determination NotesOriginally, this phage was assumed to be circularly permuted since its only close relative, Poushou, was listed as circularly permuted. The Poushou conclusion, however, was made from dubious Ion Torrent data, and later Illumina sequencing revealed that phages in this cluster indeed have defined ends and a 10-base 3' overhang. The information and final fasta file were updated in October 2018.
GC Content60.0%
Fasta file available?Yes: Download fasta file
Cluster Life CycleUnknown
Other Cluster Members

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Annotation StatusIn GenBank
Plaque NotesPlaques are 1 mm in diameter and very turbid.
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH271317
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 8
Pitt Freezer Box Grid# A8
Available Files
Plaque PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload