| Detailed Information for Phage TouchMeNot | |
| Discovery Information | |
| Isolation Host | Corynebacterium vitaeruminis CAG1 |
| Duplicates of this Phage | CloverDale |
| Found By | Kerry Iles |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Phage Hunters Integrating Research and Education |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.4375 N, 79.9465 W Map |
| Discovery Notes | This phage was isolated from the plant Impatiens pallida, collected by Dr. Tia-Lynn Ashman. The plant was washed with PYCa, filtered, and then enriched with host at 30 degrees Celsius. Purification was performed by Kira Zack. |
| Naming Notes | This phage was isolates from Impatiens pallida, a plant from the Touch-Me-Not family, Balsaminaceae. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 6, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2171 |
| Genome length (bp) | 40526 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGGCACCCAT |
| End Determination Notes | Originally, this phage was assumed to be circularly permuted since its only close relative, Poushou, was listed as circularly permuted. The Poushou conclusion, however, was made from dubious Ion Torrent data, and later Illumina sequencing revealed that phages in this cluster indeed have defined ends and a 10-base 3' overhang. The information and final fasta file were updated in October 2018. |
| GC Content | 60.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EO |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Other Cluster Members |
Click to View |
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are 1 mm in diameter and very turbid. |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewTouchMeNot_1 TouchMeNot_2 TouchMeNot_3 TouchMeNot_4 TouchMeNot_5 TouchMeNot_6 TouchMeNot_7 TouchMeNot_8 TouchMeNot_9 TouchMeNot_10 TouchMeNot_11 TouchMeNot_12 TouchMeNot_13 TouchMeNot_14 TouchMeNot_15 TouchMeNot_16 TouchMeNot_17 TouchMeNot_18 TouchMeNot_19 TouchMeNot_20 TouchMeNot_21 TouchMeNot_22 TouchMeNot_23 TouchMeNot_24 TouchMeNot_25 TouchMeNot_26 TouchMeNot_27 TouchMeNot_28 TouchMeNot_29 TouchMeNot_30 TouchMeNot_31 TouchMeNot_32 TouchMeNot_33 TouchMeNot_34 TouchMeNot_35 TouchMeNot_36 TouchMeNot_37 TouchMeNot_38 TouchMeNot_39 TouchMeNot_40 TouchMeNot_41 TouchMeNot_42 TouchMeNot_43 TouchMeNot_44 TouchMeNot_45 TouchMeNot_46 TouchMeNot_47 TouchMeNot_48 TouchMeNot_49 TouchMeNot_50 TouchMeNot_51 TouchMeNot_52 TouchMeNot_53 TouchMeNot_54 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH271317 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 6 |
| Pitt Freezer Box Grid# | D6 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |