Gordonia phage Trumpet
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Detailed Information for Phage Trumpet
Discovery Information
Isolation HostGordonia terrae 3612
Found ByCaitlyn Kline
Year Found2019
Location FoundPittsburgh, PA United States
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.443694 N, 79.954806 W Map
Discovery NotesFor every protocol after purification I used a modified protocol with 125uL of Gordonia terrae cells instead of 250 and also diluted the top agar increase the plaque size of Trumpet.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 24, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1262
Genome length (bp)50338
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCTGGGGA
GC Content66.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ1
Cluster Life CycleTemperate
Lysogeny NotesTrumpet is Virulent.
Other Cluster Members
Annotating InstitutionWashington State University
Annotation StatusIn GenBank
Plaque NotesTrumpet is growing in PYCa growth medium with Gordonia terrae host cells and incubated at 30*C for 2 days. Plaques are symmetrical and lytic with a diameter with less than 1mm in diameter.
Has been Phamerated?Yes
Gene List

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Trumpet_1
(terminase small subunit)

Trumpet_2
(terminase large subunit)

Trumpet_3

Trumpet_4
(portal protein)

Trumpet_5
(capsid maturation protease)

Trumpet_6
(major capsid protein)

Trumpet_7

Trumpet_8
(head-to-tail adaptor)

Trumpet_9
(head-to-tail stopper)

Trumpet_10

Trumpet_11
(tail terminator)

Trumpet_12
(major tail protein)

Trumpet_13

Trumpet_14
(tail assembly chaperone)

Trumpet_15
(tail assembly chaperone)

Trumpet_16

Trumpet_17
(tape measure protein)

Trumpet_18
(minor tail protein)

Trumpet_19
(minor tail protein)

Trumpet_20
(lysin A, L-Ala-D-Glu peptidase domain)

Trumpet_21

Trumpet_22
(lysin A, glycosyl hydrolase domain)

Trumpet_23

Trumpet_24

Trumpet_25

Trumpet_26

Trumpet_27
(lysin B)

Trumpet_28
(minor tail protein)

Trumpet_29

Trumpet_30
(minor tail protein)

Trumpet_31

Trumpet_32
(metalloprotease)

Trumpet_33

Trumpet_34

Trumpet_35
(helix-turn-helix DNA binding domain protein)

Trumpet_36

Trumpet_37

Trumpet_38

Trumpet_39
(tyrosine integrase)

Trumpet_40

Trumpet_41

Trumpet_42

Trumpet_43
(helix-turn-helix DNA binding domain protein)

Trumpet_44
(immunity repressor)

Trumpet_45
(helix-turn-helix DNA binding domain protein)

Trumpet_46

Trumpet_47

Trumpet_48
(antirepressor)

Trumpet_49

Trumpet_50
(excise)

Trumpet_51

Trumpet_52

Trumpet_53

Trumpet_54

Trumpet_55

Trumpet_56
(RecE-like exonuclease)

Trumpet_57
(RecT-like ssDNA binding protein)

Trumpet_58

Trumpet_59

Trumpet_60

Trumpet_61
(ASC-1 transcription coactivator)

Trumpet_62

Trumpet_63
(DNA methylase)

Trumpet_64

Trumpet_65

Trumpet_66

Trumpet_67

Trumpet_68

Trumpet_69

Trumpet_70

Trumpet_71
(RusA-like resolvase)

Trumpet_72

Trumpet_73

Trumpet_74

Trumpet_75
(helix-turn-helix DNA binding domain protein)

Trumpet_76

Trumpet_77

Trumpet_78

Trumpet_79
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMW924648
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 115
Pitt Freezer Box Grid# D10
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload