Gordonia phage Trumpet
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Detailed Information for Phage Trumpet
Discovery Information
Isolation HostGordonia terrae 3612
Found ByCaitlyn Kline
Year Found2019
Location FoundPittsburgh, PA United States
Finding InstitutionUniversity of Pittsburgh
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates40.443694 N, 79.954806 W Map
Discovery NotesFor every protocol after purification I used a modified protocol with 125uL of Gordonia terrae cells instead of 250 and also diluted the top agar increase the plaque size of Trumpet.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 24, 2020
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1262
Genome length (bp)50338
Character of genome ends3' Sticky Overhang
Overhang Length10 bases
Overhang SequenceCGGCTGGGGA
GC Content66.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterCZ
SubclusterCZ1
Cluster Life CycleTemperate
Lysogeny NotesTrumpet is Virulent.
Other Cluster Members
Annotating InstitutionWashington State University
Annotation StatusAwaiting_QC
Plaque NotesTrumpet is growing in PYCa growth medium with Gordonia terrae host cells and incubated at 30*C for 2 days. Plaques are symmetrical and lytic with a diameter with less than 1mm in diameter.
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 115
Pitt Freezer Box Grid# D10
Available Files
Plaque PictureDownload
EM PictureDownload