Arthrobacter phage TymAbreu
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Detailed Information for Phage TymAbreu
Discovery Information
Isolation HostArthrobacter sp. ATCC 21022
Found ByEmma Sweet and Jessica Dahlke
Year Found2013
Location FoundHudson, WI USA
Finding InstitutionUniversity of Wisconsin-River Falls
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates44.984444 N, 92.754444 W Map
Discovery NotesSoil sample was collected at 9:30am on 9-21-13 from a tomato garden approximately 6 inches from a tomato plant and taken from the depth of 30mm. The air temperature at the time of collection was 66 degrees F and the weather was sunny and dry. The soil was amended with compost and was dry and loose. After the enrichment process, we discovered two different sized plaques. Emma chose to isolate the small plaques while Jessica chose to isolate the bigger plaques. After a few isolations processes, the plaques started to vary in size. We then carried through all the experiments and discovered that our phage genomic DNA and electron microscope results were the same.
Naming NotesWhen Emma and Jessica still thought that they were isolating different phages, Emma chose to name her phage Tyma, a mixture of her and her boyfriend's name, and Jessica chose to name her phage Abreu, after her high school Biology teacher. After discovering the phage was the same, we combined the two names to create TymAbreu.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 16, 2013
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage267
Genome length (bp)15556
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCCCGCGCCACC
GC Content60.1%
Fasta file available?Yes: Download fasta file
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesPlaques are clear with no halo and range in size from 1mm-2mm.
Number of Genes26
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKT783672
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 4.4 x 10^9
Date of SEA Lysate Titering Oct 15, 2013
Pitt Freezer Box# 1
Pitt Freezer Box Grid# B9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
Virtual Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload