Gordonia phage Vagabundus
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Detailed Information for Phage Vagabundus
Discovery Information
Isolation HostGordonia terrae CAG3
Found ByDebbie Reck
Year Found2022
Location FoundCullowhee, NC United States of America
Finding InstitutionWestern Carolina University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature21°C
GPS Coordinates35.311667 N, 83.189167 W Map
Discovery NotesThe sample was collected on Western Carolina University's trail system, near the beginning of the trail. Collected on 10/05/2022 in the mid-afternoon and the temperature was 70 degrees Fahrenheit.

Sample was found in a hollow tree-root, exposed to the elements. I didn't have to dig deeper to get to fine soil and to collect it. It was also in a shaded region.

The soil was fine in texture, no large clumps. Very dark brown in color. Soil was also a little damp. There were twigs, leaves, and other forest debris inside the root.
Naming NotesI named my bacteriophage Vagabundus for two reasons. One, Vagabundus is Latin for Vagabond and Latin, to me, just sounds so cool and I felt as though it had a nice ring to it.

The other reason is that for about 6-7 weeks, I was having no luck in finding a positive phage sample. When I mapped all of the locations I had been to, the map showed that I had traveled all around the campus (not everywhere, but I covered quite some distance). On the day that I found my phage sample (unbeknownst yet) I decided to go to the campus's trail system and wander about. As I was looking and walking around, I stumbled upon this exposed tree root, and I felt as though it were calling to me, saying that my sample was here; and sure enough, it was!

*Vagabond means to wander from place to place (without a set home or job). I felt as though I had wandered about my campus and the trail system, trying to locate my phage.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 6x10^8 PFU/mL
Pitt Freezer Box# 153
Pitt Freezer Box Grid# C2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload