Detailed Information for Phage William |
Discovery Information |
Isolation Host | Gordonia terrae 3612 |
Found By | Parampreet Deol |
Year Found | 2016 |
Location Found | Pittsburgh, PA USA |
Finding Institution | University of Pittsburgh |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 40.443383 N, 79.955009 W Map |
Discovery Notes | The soil was collected from a flower bed outside a clear, sunny day. The soil was slightly moist, and I dug about 3 inches into the ground to collect it. Afterwards, it was stored in a refrigerator for about 1 day before being processed in lab. |
Naming Notes | The name for this phage comes from the location it was found. It was found outside of the William Pitt Union at the University of Pittsburgh. |
Sequencing Information |
Sequencing Complete? | Yes |
Date Sequencing Completed | Feb 16, 2017 |
Sequencing Facility | Pittsburgh Bacteriophage Institute |
Shotgun Sequencing Method | Illumina Sequencing |
Approximate Shotgun Coverage | 456 |
Genome length (bp) | 50678 |
Character of genome ends | 3' Sticky Overhang |
Overhang Length | 10 bases |
Overhang Sequence | TCGCCGGTGA |
GC Content | 66.5% |
Sequencing Notes | There were two SNPs in this sample.
At position 23844, ~40% of the reads showed a G instead of the consensus A. At position 24218, ~35% of the reads showed a G instead of the consensus A. |
Fasta file available? | Yes: Download fasta file |
Characterization |
Cluster | CV |
Subcluster | -- |
Cluster Life Cycle | Temperate |
Other Cluster Members |
|
Annotating Institution | University of Pittsburgh |
Annotation Status | In GenBank |
Plaque Notes | William produces clear plaques the relatively round and small, ranging from approximately 1 mm to 3 mm in length. |
Morphotype | Siphoviridae |
Has been Phamerated? | Yes |
Gene List |
|
Submitted Minimal DNA Master File | Download |
Publication Info |
Uploaded to GenBank? | Yes |
GenBank Accession | MK801721 |
Refseq Number | None yet |
Archiving Info |
Archiving status |
Archived |
Pitt Freezer Box# |
42 |
Pitt Freezer Box Grid# |
H12 |
Available Files |
Plaque Picture | Download |
Restriction Digest Picture | Download |
EM Picture | Download |
GenBank File for Phamerator | Download |