Mycobacterium phage YoyoKar
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| Detailed Information for Phage YoyoKar | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Gia Sohi, Yonal Karandana |
| Year Found | 2025 |
| Location Found | Las Vegas, NV United States |
| Finding Institution | University of Nevada Las Vegas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 25°C |
| GPS Coordinates | 36.243333 N, 115.325278 W Map |
| Discovery Notes | The sample was collected near the roots of a potted green chili plant, with a 31°C outside temperature, 18% humidity, and 11 mph wind. |
| Naming Notes | Named after the people who found the phage. "Yoyo" refers to Yonal and "Kar" refers to Gia. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 15, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 738 |
| Genome length (bp) | 70883 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 4 bases |
| Overhang Sequence | GTCT |
| GC Content | 65.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | O |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | University of Nevada Las Vegas |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Turbid and cloudy plaques with faint halos around them. Average plaque size is 0.2 mm. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 2.7 x 10^10 PFU/mL |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
