Mycobacterium phage Agent47
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Detailed Information for Phage Agent47
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByKirin Guay, Jacob Bunszel
Year Found2023
Location FoundOrono, ME United States of America
Finding InstitutionUniversity of Maine, Honors College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature20°C
GPS Coordinates44.8966 N, 68.65925 W Map
Discovery NotesBlueberry bush growing in a garden bin, collected around 6-8" in depth.
Naming NotesNamed after the main character in the Hitman video game series, reflecting the bacterial-killing nature of a bacteriophage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 16, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage751
Genome length (bp)61143
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceCTCAGTGGCAT
GC Content65.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterK
SubclusterK5
Cluster Life CycleTemperate
Other Cluster Members

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ActinUp (K1)

Adephagia (K1)

Adonis (K1)

Ageofdapage (K1)

AlishaPH (K1)

Amelie (K1)

Anaya (K1)

Angelica (K1)

Apocalypse (K1)

Asayake (K1)

Atiba (K1)

BaghaKamala (K1)

BarrelRoll (K1)

BEEST (K1)

Beezoo (K1)

Bella96 (K1)

Belladonna (K1)

BGlluviae (K1)

Biglebops (K1)

Blizzard (K1)

Boiiii (K1)

Capricorn (K1)

CaseJules (K1)

CheetoDust (K1)

Chris (K1)

CREW (K1)

CrimD (K1)

Curiosium (K1)

Dalmuri (K1)

Deby (K1)

Devera (K1)

Dole (K1)

DrHayes (K1)

Durfee (K1)

Efra2 (K1)

Emerson (K1)

Enkosi (K1)

Ganymede (K1)

Geraldini (K1)

Guanica15 (K1)

HedwigODU (K1)

Homura (K1)

Hyperbowlee (K1)

Illumine (K1)

Inky (K1)

Jarvi (K1)

JAWS (K1)

Jayhawk (K1)

Jeckyll (K1)

Joy99 (K1)

KiSi (K1)

LastHope (K1)

LaterM (K1)

LeMond (K1)

LilPharaoh (K1)

LindNT (K1)

Llorens (K1)

Lunahalos (K1)

MarkPhew (K1)

Mdavu (K1)

MeaningOfLife (K1)

Megsy (K1)

Murucutumbu (K1)

Mynx (K1)

Nibb (K1)

Nikao (K1)

Niklas (K1)

Nutello (K1)

Oscar (K1)

Padfoot (K1)

Padpat (K1)

Peanam (K1)

Peel (K1)

Piatt (K1)

Pokerus (K1)

Prithvi (K1)

QuincyRose (K1)

Ramen (K1)

Rapunzel97 (K1)

Rosmarinus (K1)

SamuelLPlaqson (K1)

Scarlett (K1)

SgtBeansprout (K1)

Shaobing (K1)

ShiaSurprise (K1)

Slimphazie (K1)

Spock (K1)

Stinson (K1)

Sulley (K1)

Tachez (K1)

TaiwanKao (K1)

TiniBug (K1)

Tiri (K1)

Topper (K1)

TreyKay (K1)

TruffulaTree (K1)

Twitch (K1)

Urkel (K1)

Validus (K1)

Veliki (K1)

YoureAdopted (K1)

Yunkel11 (K1)

Zabiza (K1)

Zavala (K1)

BoostSeason (K2)

DismalFunk (K2)

DismalStressor (K2)

Findley (K2)

Marcoliusprime (K2)

Milly (K2)

Mufasa (K2)

Strobilo (K2)

TM4 (K2)

ZoeJ (K2)

Hurricane (K3)

Keshu (K3)

MacnCheese (K3)

Pharb (K3)

Pixie (K3)

ShedlockHolmes (K3)

TBond007 (K3)

TribleTrouble (K3)

Chancellor (K4)

Cheetobro (K4)

Eponine (K4)

Fionnbharth (K4)

JF1 (K4)

Juliette (K4)

Malthus (K4)

MissDaisy (K4)

Mitti (K4)

OmniCritical (K4)

Patt (K4)

Reptar3000 (K4)

Ruthiejr (K4)

SamScheppers (K4)

Slarp (K4)

Taquito (K4)

Wintermute (K4)

Agent47 (K5)

AlleyCat (K5)

Collard (K5)

Dadosky (K5)

Edugator (K5)

Feyre (K5)

Gengar (K5)

Guillsminger (K5)

Heftyboy (K5)

InvictusManeo (K5)

Kratio (K5)

Larva (K5)

Leston (K5)

Miryou (K5)

OkiRoe (K5)

Omnicron (K5)

Paola (K5)

Psycho (K5)

Rando14 (K5)

SoSeph (K5)

Thyatira (K5)

Waterfoul (K5)

Amgine (K6)

Amohnition (K6)

Bryler (K6)

Cain (K6)

DarthP (K6)

Ekdilam (K6)

Ellie (K6)

Fefferhead (K6)

Hammy (K6)

Krueger (K6)

Marshawn (K6)

PhelpsODU (K6)

Phrank (K6)

Shadow1 (K6)

SirPhilip (K6)

Sunflower1121 (K6)

Syra333 (K6)

TClif (K6)

Tierra (K6)

Unicorn (K6)

Ximenita (K6)

Yuna (K6)

Aminay (K7)

Boilgate (K8)

Annotating InstitutionUniversity of Maine, Honors College
Annotation StatusQC Complete
Plaque NotesPlaques are 1-3mm in diameter, small slightly cloudy plaques with clear plaque centers.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Agent47_1

Agent47_2

Agent47_3

Agent47_5

Agent47_6
(terminase)

Agent47_7
(portal protein)

Agent47_8
(capsid maturation protease)

Agent47_9

Agent47_10
(scaffolding protein)

Agent47_11
(major capsid protein)

Agent47_12
(head-to-tail adaptor)

Agent47_13
(head-to-tail stopper)

Agent47_14

Agent47_15
(tail terminator)

Agent47_16
(major tail protein)

Agent47_17
(tail assembly chaperone)

Agent47_18
(tail assembly chaperone)

Agent47_19
(tape measure protein)

Agent47_20
(minor tail protein)

Agent47_21
(minor tail protein)

Agent47_22

Agent47_23
(minor tail protein)

Agent47_24

Agent47_25

Agent47_26

Agent47_27

Agent47_28

Agent47_29
(lysin A)

Agent47_30
(lysin B)

Agent47_31
(holin)

Agent47_32

Agent47_33
(helix-turn-helix DNA binding domain)

Agent47_34
(metallophosphatase)

Agent47_35

Agent47_36

Agent47_37
(helix-turn-helix DNA binding domain)

Agent47_38
(hydrolase)

Agent47_39
(serine integrase)

Agent47_40

Agent47_41

Agent47_42
(immunity repressor)

Agent47_43
(helix-turn-helix DNA binding domain)

Agent47_44

Agent47_45

Agent47_46

Agent47_47

Agent47_48

Agent47_49

Agent47_50

Agent47_51

Agent47_52

Agent47_53

Agent47_54

Agent47_55
(Cas4 exonuclease)

Agent47_56

Agent47_57

Agent47_58

Agent47_59

Agent47_60
(NrdH-like glutaredoxin)

Agent47_61
(HNH endonuclease)

Agent47_62
(HNH endonuclease)

Agent47_63
(DNA primase/polymerase/helicase)

Agent47_64
(DNA helicase)

Agent47_65
(Holliday junction resolvase)

Agent47_66
(helix-turn-helix DNA binding domain)

Agent47_67

Agent47_68

Agent47_69
(helix-turn-helix DNA binding domain)

Agent47_70

Agent47_71

Agent47_72

Agent47_73

Agent47_74

Agent47_75

Agent47_76
(RNA ligase)

Agent47_77

Agent47_78

Agent47_79

Agent47_80

Agent47_81

Agent47_82

Agent47_83

Agent47_84

Agent47_85
(dpdA-like tRNA-guanine transglycosylase)

Agent47_86
(toxin in toxin/antitoxin system, BrnT-like)

Agent47_87
(antitoxin in toxin/antitoxin system, BrnA-like)

Agent47_88

Agent47_89

Agent47_90

Agent47_91

Agent47_92

Agent47_93

Agent47_94
(helix-turn-helix DNA binding domain)

Agent47_95

Agent47_96

Agent47_97
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 186
Pitt Freezer Box Grid# E9
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload