| Detailed Information for Phage GreaseLightnin |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Richard Stauffer and Nic Bussian |
| Year Found | 2013 |
| Location Found | Kenosha, WI USA |
| Finding Institution | Carthage College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 42.6194 N, 87.8212 W Map |
| Discovery Notes | The soil sample was taken from underneath a tree next to a campus building, underneath some leaves and wood chips. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 22, 2015 |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 8284 |
| Genome length (bp) | 48424 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | CCCGCCCCCCGA |
| GC Content | 67.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | P |
| Subcluster | P1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Carthage College |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque morphology, after incubated for 48 hours, consists of each plaque having a diameter of approximately 1 mm, each plaque is clear, and there was no variation in this plaque morphology. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK494122 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
6x10^9 |
| Pitt Freezer Box# |
4 |
| Pitt Freezer Box Grid# |
H2 |
| Available Files |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
