| Detailed Information for Phage Halley |
| Discovery Information |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Aislinn Rowan |
| Year Found | 2011 |
| Location Found | Bethlehem, PA USA |
| Finding Institution | Lehigh University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.603055 N, 75.375278 W Map |
| Discovery Notes | Soil was taken from below a runoff rainwater stream and was thus very damp. It was taken from approximately 1 inch below the surface and contained a few small rocks. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 4, 2012 |
| Genome length (bp) | 112351 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 4 bases |
| Overhang Sequence | ATCC |
| GC Content | 60.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | J |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Lehigh University |
| Annotation Status | In GenBank |
| Plaque Notes | At 24 hours: small (approx. 1 mm), round, turbid
At 48 hours: larger (approx. 3mm), round, very small clear center inside a thick turbid ring
*At 48 hours there are also smaller 1-2mm turbid plaques; smaller plaques were always picked for streaks and titers and the same dual morphology appears at 48 hours. Comparison titers made from picking small and large plaques separately also show identical morphology development.* |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH077579 |
| Refseq Number | None yet |
| Published in Paper | Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity |
| Archiving Info |
| Archiving status |
Archived |
| SEA Designator |
2011LEHIhalleyADR |
| Pitt Freezer Box# |
25 |
| Pitt Freezer Box Grid# |
63 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |