Question of the Month - December 2012/ January 2013
Dec 17, 2012
Happy New Year! Before we move to the dark side of bacteriophages, the bioinformatic side, I thought one more question about plaque morphology could be appropriate. Here is a photo of plaques (on M. smeg) of Mycobacteriophage Spartacus that have been incubated for 5 days. After 24 hours of incubation, there were ~ 40 cloudy plaques, each with an estimated diameter of about 3mm. Here, at 5 days, you see a central clearer zone of lysis, with a surrounding cloudy area that at times measures at least 1 cm . In addition, there is a spray of tiny clearer plaques (indicated by red arrows) emanating from the center of the original plaque, but typically in only one direction. Can you explain this morphology?