Arthrobacter phage ChamaMama
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Detailed Information for Phage ChamaMama
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Found BySana Haque
Year Found2023
Location FoundLombard, IL U.S.A.
Finding InstitutionBenedictine University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature22°C
GPS Coordinates41.866389 N, 87.996389 W Map
Discovery NotesThe phage Chama Mama is a lytic phage with a plaque diameter of 0.8mm. A clonal population of this phage was not obtained throughout the course of this research, even after three rounds of purification. Chama Mama also has DNA with no restriction sites for the restriction enzyme NspI. This phage produces accurate serial dilutions, but a high concentration of phage is needed to create a webbed plate.
Naming NotesA few close friends and I traveled to New York City to celebrate our high school graduations. On our trip, we visited a restaurant called Chama Mama because one of my friends really wanted to try it. After our meal, we were still starving due to the tiny portions that the restaurant provided. The name of my plaque is in dedication to this restaurant; although it may not have been an enjoyable meal, something good could still come from my friend's horrible dining suggestion.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThis phage produces a mixture of plaque sizes. After performing 3 rounds of purification, a clonal population was still not obtained due to at least two plaque diameters present on the plate. These sizes include some plaques with a 0.8mm diameter and some with a 0.2mm diameter. The original Chama Mama plaque size was 0.8mm, and this is the more prevalent size in most plates.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload