Mycobacterium phage Gingersnap5
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| Detailed Information for Phage Gingersnap5 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Jackson C. Smith |
| Year Found | 2021 |
| Location Found | Bowling Green, KY United States |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.986917 N, 86.454139 W Map |
| Discovery Notes | The soil was found right outside Florence Schneider Hall. The soil was very rough, full of mulch and insects. When I gathered the sample, it was raining so the sample was damp and a pool of water was visible. |
| Naming Notes | The name of this phage was decided on a coin flip. It was either going to be Lolipop7 or Gingersnap5 |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | Gingersnap5 appears to be a very temperate phage. This is noticeable by the differences in the appearance of the plaques based on how long they had been exposed to the phage. Plaques that had been exposed for a shorter amount of time were often completely cloudy while more mature plaques formed halos. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Gingersnap5 produces very turbid plaques with visible "halos". These halos can be seen more clearly in more mature plaques. The inner part of the plaques appear mostly clear while the outer ring/halo is more cloudy, indicating less lysed cells. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.29*10^10 pfu/mL |
| Pitt Freezer Box# | 137 |
| Pitt Freezer Box Grid# | G9 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
