Microbacterium phage Grog
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| Detailed Information for Phage Grog | |
| Discovery Information | |
| Isolation Host | Microbacterium paraoxydans NRRL B-24275 |
| Found By | Ayden Pyle |
| Year Found | 2023 |
| Location Found | Cullowhee, NC United States of America |
| Finding Institution | Western Carolina University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 35.310248 N, 83.187476 W Map |
| Discovery Notes | Found in a shady spot under a bridge near a creek. The sample was from right along the water edge, so the soil was wet and somewhat gritty. It also had a lot of minerals. |
| Naming Notes | I retrieved my soil sample from under a bridge, so I thought it would be fitting to give my phage a troll-like name. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Originally the plaques were very small and uniform. After I was done purifying it and was in the process of growing a stock, the morphology changed dramatically. I purified it again, but the morphology of the plaques remained the same. My instructor thinks it has something to do with her making a new batch of bacteria stock, and my phage being particularly sensitive to the change. There was another similar case in the class. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 10^10 PFU/mL |
| Pitt Freezer Box# | 175 |
| Pitt Freezer Box Grid# | F5 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
