Mycobacterium phage JayJo04
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Detailed Information for Phage JayJo04
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByJayson Faulkner & Josiah Harris
Year Found2023
Location FoundAtlanta, NC United States
Finding InstitutionMorehouse College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS CoordinatesUnavailable
Discovery NotesWe want to describe where we fond out soil phage sample and why the soil was wet. Our model was collected from the area surrounding Merrill Hall, a prominent location was moist because the groundskeeper practiced regular irrigation to keep the nearby plants and greenery healthy. This constant watering creates a unique microenvironment in the soil, where moisture levels fluctuate regularly.

Our decision to study a phage sample from this area was motivated by the intriguing relationship between soil moisture and the organisms that call this environment home. We are interested in understanding how soil-dwelling phages, viruses that infect bacteria, interact with the microbial communities that thrive in response to these moisture variations. By investigating this particular soil phage sample, we hope to uncover influence the dynamics of these tiny but influential players in the soil ecosystem. This research provides a gateway to better comprehending the intricate relationships between phages, bacteria, and their environment, shedding light on the broader ecological implications of soil health and vitality.
Naming NotesOur phage's name, JayJo4, holds a special significance that reflects our unique connection to the research project. The first part, "JayJo," is a combination of the initial letters of our names, symbolizing the collaborative effort and shared dedication to this investigation. This choice emphasizes the collective nature of scientific discovery, highlighting the teamwork that underpins our research.


The second part, "04," is particularly meaningful represents our birth year. The Greek letter "theta" (θ) in the name stands for the fourth letter of the Greek alphabet, signifying the numerical representation of "4." By incorporating this reference to our birth year, we infuse a personal touch into our phage's nomenclature, making it uniquely our own and reinforcing the connection between our individual identities and our scientific pursuits. In doing so, we honor the essence of teamwork and the personal investment that each of us brings to this exciting journey of discovery.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesPhage JayJo04 displays distinct plaque morphology when grown on a bacterial lawn. Plaques are the clear, circular regions on a bacterial lawn where the phage has infected and killed the bacterial cells.
These observations provide
valuable insights into the characteristics and behavior of our phage.

1. Plaque Size: The plaques formed by JayJo04 are generally small in size, typically ranging from 1 to 2 millimeters in diameter. This suggests that the phage might not have a high burst size, which is the number of phage particles released from an infected bacterial cell upon lysis.

2. Plaque Shape: The plaques exhibit a
round and well-defined shape. Their
Circular appearance suggests that JayJo@4 has a uniform and consistent infectivity pattern on the bacterial host.

3. Clarity: The plaques are characterized by a clear center, which indicates complete lysis of the bacterial cells within the plaque. This suggests that JayJoe4 has strong lytic capabilities against its host bacteria.

4. Halo Formation: A faint halo can be observed around some of the plaques.
This halo signifies a zone of inhibited bacterial growth, likely due to the release of antimicrobial compounds by the phage during its infection cycle.

5. Plaque Density: Plaques formed by JayJoθ4 are moderately dense across the bacterial lawn. This density may indicate that the phage has a relatively moderate replication rate.

These observations of plaque morphology provide valuable information about the behavior and characteristics of our phage JayJoθ4.

We made a noteworthy discovery in our exploration of phage JayJo04's plaque morphology. Among the plaques formed,
three distinct sizes were measured and
recorded. These plaque dimensions are 20.22 um, 2179.17 um, and 2211.22 um.
Such variation in plaque size among the observed plaques suggests potential genetic diversity within the phage population. This raises intriguing questions about the genetic factors contributing to this variation. This finding adds an exciting dimension to our investigation, prompting further inquiry into the genetic and biological aspects of phage JayJoθ4.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives