Mycobacterium phage Nab123
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| Detailed Information for Phage Nab123 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | SoHye Jang and Natalie Matthews |
| Year Found | 2017 |
| Location Found | Pullman, WA USA |
| Finding Institution | Washington State University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 46.7275 N, 117.1639 E Map |
| Discovery Notes | Soil sample found at WSU campus near Gannon Goldsworthy dorms. The soil was found next to the cement in a grass, patchy area. The conditions it was found in was below ice and snow. |
| Naming Notes | We named it Nab123 because it is slang for capture. When we were collected soil our main goal was to capture phage when we did our experiments in lab. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Our plaque assay results for 2/9/17: Phage is not diluted enough. Thus, doing a streak assay from original spot test and to do six dilutions. For the streak assay, taking a wood stick and wiped the spot from the spot test. We then took a new agar plate and wiped in different directions making sure each was connecting with streaks. We then set for ten minutes in aseptic conditions and then taped the sides. The we incubated for twenty four hours and then was put in the refrigerator. Our plaque assay results for 2/16/17: The phage became lyctic and then we conducted our first streak assay. This process included taking our most isolated phage and using a clean wooden stick, touching the phage and taking an agar plate and making three different streaked sections connecting with one distinct streak connecting them. Then we marked the area we drew our phage from and labeled our new agar plate with what procedure we followed through with. Then we left the agar plate in aseptic conditions for ten minutes and then incubated for twenty -four hours and then was incubated for the remaining time in the refrigerator. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 63 |
| Pitt Freezer Box Grid# | C6 |
| Available Files | |
| Plaque Picture | Download |
