Mycobacterium phage Olaf
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| Detailed Information for Phage Olaf | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Nicole Joseph and Martha Cabrera |
| Year Found | 2014 |
| Location Found | Spokane, WA USA |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 47.6672 N, 117.402 W Map |
| Discovery Notes | We found Olaf in a flower bed behind College Hall. Our soil was colored of dark and light brown. The weight of the sample we used was 1.202 grams. The weather on the day it was collected was -1 degrees Celsius with a humidity of 79%. We cultured our enrichment at room temperature of 25 degree celsius. Our phage grows at 25 degree celsius but it didn't grow at 37 degrees celsius. |
| Naming Notes | The movie Frozen, Olaf is the snowman. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | Temperate |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Olaf is a temperate phage. The capsid diameter is 80nm The tail length is 225nm The total length is 305nm |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.5 x 10^8 |
| Date of SEA Lysate Titering | Feb 28, 2014 |
| Pitt Freezer Box# | 17 |
| Pitt Freezer Box Grid# | A4 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |