Arthrobacter phage OswaldTheFirst
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| Detailed Information for Phage OswaldTheFirst | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Andrew Levy |
| Year Found | 2021 |
| Location Found | Los Angeles, CA United States |
| Finding Institution | University of Southern California |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.018928 N, 118.2861 W Map |
| Discovery Notes | - Time: 11:28 AM on September 16, 2021 - Temperature: 70° F (feels like 70° F) - What The Location Looks Like: Thin tree by the George Tirebiter statue outside of Zumberge Hall at the University of Southern California (about 50 ft from the building). This statue sits in the middle of Trousdale Parkway on the southern end of campus. The day is sunny, the skies are clear, and there is ample shade on the soil provided by the nearby tree. On both sides of the collection site, there is brick that makes up Trousdale Parkway. Droves of students walk along Trousdale Parkway (and thus past the site) every day, and there is a street about 250 ft south of the collection site. - How far Below the Surface: ~7.50 cm or 2.95 in - UV Index: 7 - Air Quality: 78 - Moderate - Humidity: 59% - Wind: wsw 1 mph - Chance of Rain: 10% - Pressure: 29.87 inHg - Precipitation: 0 in - Visibility: 6 mi - Soil Condition: The soil is fairly moist, it is next to a thin tree, it is dark brown in color, and it is more moist at lower depths. The soil is easy to break up, and it does not clump together when dug into (powdery yet still moist in texture). Small parts of the nearby tree (twigs, leaves, etc.) have fallen from the tree and are spread throughout the top of the soil. There are small rocks and green plant life throughout the collection site. There is a small piece of glass embedded near the collection site (most likely a result of littering). A plastic spoon was used to break up and extract the soil. |
| Naming Notes | In the popular video game Red Dead Redemption 2, which was released in 2018, you play as an outlaw during the end of the cowboy era, when America was attempting to tame the Wild West and bring it into modernity. In this game, you get to own your own horse, name that horse, and form a strong bond with that horse throughout the course of the game. In the game, I decided to name my horse Oswald I (just in case he died and I needed an Oswald II). Luckily, Oswald I remained with me throughout the entirety of the game, and we formed a connection that any cowboy would surely be jealous of. In honor of this trusty steed, I decided to name my bacteriophage Oswald I. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques are quite turbid, making them difficult to see on the agar plate. Because these plaques are so turbid/cloudy, they are most likely the result of a temperate phage, which mainly the lytic cycle on the plate. The plaques are very circular and relatively small (about 1-2 mm in diameter). |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 7 x 10^9 pfu/mL |
| Pitt Freezer Box# | 134 |
| Pitt Freezer Box Grid# | E9 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
