Mycobacterium phage Pancakey
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| Detailed Information for Phage Pancakey | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Burke and Altamore |
| Year Found | 2023 |
| Location Found | Easton, PA USA |
| Finding Institution | Lafayette College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | 40.69896 N, 75.21107 W Map |
| Discovery Notes | Collected from a grassy area from a smaller campus quad (by Waston Hall). Soil was undisturbed and collected around 2 inches deep. It was 5.5 degrees(celsius) when the sample was collected, roughly 2 yards from the side walk. Collected on 1/24/23 |
| Naming Notes | Our phage was consistently on the larger side, so we felt that "Pancakey" was a fun and endearing way to acknowledge its size |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Our phage presented in two different sizes. We chose to proceed with the larger spot (about 2mm) after three rounds of serial dilutions as it was more isolated. Changed from lytic to lysogenic. Three rounds of serial dilutions were performed to ensure a purified sample. Final dilution was made to our phage DNA solution for a satisfactory restriction digestion preparation. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |