Mycobacterium phage Platypus
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| Detailed Information for Phage Platypus | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Chelsea Miller, Anthony Terra, Chloe Oscar-Phoenix |
| Year Found | 2023 |
| Location Found | Unavailable |
| Finding Institution | Merrimack College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | Unavailable |
| Discovery Notes | The sample was collected at approximately 42.41868° N, -71.14660° W, in the backyard of 12 Webcowet Road in Arlington, MA. Gloves were donned before touching the soil, and a nearby stick was used to dig about 4 inches into the ground. This was at 9:10 am on 9/11/2023. It was warm and humid out, having just rained. The soil was damp and was more wet when dug into. About a foot away from where the sample was collected there are two larger plants, with smaller plants and grass closer by. After digging, the soil was collected with clean, dry gloves and placed into a clean, dry plastic bag. The sample was used later that morning. Figure 1. Location of sample collection in backyard (left) and on a map (right) |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Generation 3 was used to create Generation 4. Before this, previous generations were used to create subsequent generations. As shown in Figure 5, the plaques were distributed as expected and many plaques were yielded. They were clear with clear margins, meaning they were likely in the lytic cycle. They had a homogeneous appearance and were about 0.27 mm in diameter. Figure 3 shows that plaque that was chosen to be picked from Generation 3 in the 10-5 dilution. The plate only had about seven plaques and they were spread around the edges of the plate. However, the results shown for Generation 4 (Figure 5) were not expected. Generation 4 yielded only about six plaques in total and they were all on the 100 plate. None were found on either other plate. These plaques were clear with cloudy margins. They are homogenous with a uniform appearance. The plaques were distributed through the middle of the plate. They were each about 0.38 mm in diameter, though some were smaller. As the plaques are mostly clear, they are in the lytic cycle as well. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 182 |
| Pitt Freezer Box Grid# | C7 |
| Available Files | |
| Plaque Picture | Download |
