Mycobacterium phage Saitama
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Detailed Information for Phage Saitama
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByAnastasia Guseva and Breanna Edinger
Year Found2016
Location FoundCorpus Christi, TX USA
Finding InstitutionDel Mar College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature37°C
GPS CoordinatesUnavailable
Discovery NotesThe soil/medium in which the phage was collected from was extracted on August 29th, 2016 at precisely 12:21 p.m. The area was near a drainage close to a building at the college, however, it was enclosed and nearly completely shaded with the shrubbery and plant-life that adorned the area. It was secluded and quite dark with very little light penetration. The weather at the time was mostly cloudy with a humid-type of air quality. The temperature was hot with a temperature of 28.9 degrees Celsius (84 degrees Fahrenheit). The soil itself was dark and dry with a clay-like texture that clumped together in small solids.
Naming NotesThe reason the name was chosen was due to the fact that the general physical appearance of the phage EM image were similar to a character my partner and I both knew. Both shared the similarities of a round, almost bald-like shape of a head that was more noticeable than the rest of their forms. They both tend to blend in, though in the case of the phage that is mostly due to lingering debris in the image rather than an official characteristic.
Sequencing Information
Sequencing Complete?No
Sequencing FacilityUniversity of North Texas
Shotgun Sequencing MethodSanger Sequencing
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Lysogeny NotesThe plaques appeared to be lysogenic since the plaques had appeared turbid (not transparent), therefore, the cell hadn't been completely destroyed, a factor that would set it aside as lytic.
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesThe phage plaques were small with a slightly opaque form. They appear as dots or spots and rarely deviated in size unless clumped together. The small size of the plaques might be due to less phage or simply because of a characteristic of our phage singularly.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 34
Pitt Freezer Box Grid# H5
Available Files
Plaque PictureDownload
EM PictureDownload