Gordonia phage Scootypjr
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| Detailed Information for Phage Scootypjr | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Former names | None |
| Found By | Michael J. Lauer |
| Year Found | 2017 |
| Location Found | Pittsburgh, PA USA |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 40.444034 N, 79.956081 W Map |
| Discovery Notes | The phage was discovered in a garden pot (in soil) outside of Nordenberg hall on the University of Pittsburgh campus. This pot is right outside of the entrance to the freshman dorms in Nordenberg hall, facing Soldier's and Sailors on the Pitt campus. This pot is white, and contains a large amount of soil in volume. See lab E-notebook for an image. |
| Naming Notes | Scootypjr comes from an episode of FOX's show "Futurama"in which they give the main character a "safe" kid's hover-bike that floats above the ground. It's modo is "who's ready for some safe fun?," which is perfect in the case of discovering and characterizing a new bacteriophage. The bike looks like a little puff, similar to what the plaques and actual electron micrograph of the phage turned out to look like. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | Lysogen testing has not been conducted to measure host range, however, plaques are very turbid, suggesting lysogenic behavior of the phage. The phage infects Gordonia terrae as a given. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Plaques obtained ranged from 1.5 mm to 2.5 mm. They were fairly small and very turbid compared to other phages. The plaques are actually very difficult to see due to their cloudy nature. Many diluted plates contained spots that were very spaced out. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 3.666x10^11 pff/mL |
| Date of SEA Lysate Titering | Feb 27, 2017 |
| Pitt Freezer Box# | 56 |
| Pitt Freezer Box Grid# | C10 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
