Mycobacterium phage TwoLucky
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| Detailed Information for Phage TwoLucky | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Emily Ezra and Colleen Mitchell |
| Year Found | 2014 |
| Location Found | Spokane, WA USA |
| Finding Institution | Gonzaga University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 47.668056 N, 117.4025 E Map |
| Discovery Notes | -Collected soil sample from the roots of a green bush in a garden bed near College Hall, a historical building on campus. -Collected at night on January 27, 2014 -Isolated one phage after many series of dilutions and plaque streaking assays. |
| Naming Notes | -We choose TwoLucky because our first sample did not contain any phage to infect the smeg, therefore we had to try again with a different environmental sample. -We were lucky our second time finding phage in a soil sample. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | -Our plaques are small in size, clear in turbidity, and rough around the edges. -They grow very rapidly to produce pin prick looking plaques in a short amount of time. -No plaque growth is seen when the petri dish is place at 37 degrees Celsius, but is seen at room temperature. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 17 |
| Pitt Freezer Box Grid# | G1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
