Mycobacterium phage WonderFalls
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Detailed Information for Phage WonderFalls
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMolly Diamond
Year Found2012
Location FoundPullman, WA Whitman
Finding InstitutionWashington State University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates46.730094 N, 117.178467 W Map
Discovery NotesCollected 9-11-12. Air temperature 63?F. Sample taken from just beneath the surface of a gravely area in Downtown Pullman. Soil was by a path and manmade waterfall. Ground was moist.
Naming NotesI first nicknamed my phage "falls" because I found them near a manmade waterfall. One day in lab I accidentally froze my spot test. I panicked and redid the spot test, but I also put the frozen spot test in the incubator, just in case. I thought if the frozen plate produced plaques, it would be a wonder phage. Plaques grew and I was very proud, so they're my wonder plaques. Wonder Falls was also the name of an obscure TV show my mother and I used to watch, so it is partially an homage. I think the name fits.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Annotation StatusNot sequenced
Plaque NotesMy morphology changed from the medium titer lysate to the high titer lysate.
My plaques are round and clear. They produce both large and small plaques. At first they just have shadows of halos, but if you leave them out they grow to bullseyes. After the high titer lysate they no longer grew halos.
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2012WASUwonderfallsMMD
SEA Lysate Titer 1.55 x 10^9
Date of SEA Lysate Titering Nov 13, 2012
Pitt Freezer Box# 42
Pitt Freezer Box Grid# 50
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload