Mycobacterium phage ACE15
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| Detailed Information for Phage ACE15 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | K. Gilbert, M. Osterndorf, M. Bertels, B.Flanagon |
| Year Found | 2015 |
| Location Found | Albion, MI USA |
| Finding Institution | Albion College |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 84.742457 N, 42.240537 E Map |
| Discovery Notes | A soil sample was obtained from the grounds of Albion College, and a suspension of it was centrifuged in order to separate the components by density. The liquid supernatant was extracted from the centrifuged material. We used serial dilutions of supernatant to start growing phages in smeg cultures, using both further series of dilutions and streaking to isolate a single strain of phage, which was extracted into a medium titer lysate. Once this was completed, we did a gel electrophoresis and used that sample to extract DNA and obtained an electron micrograph of individual virions. |
| Naming Notes | Albion College Equestrian year 2015 |
| Sequencing Information | |
| Sequencing Complete? | No |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | 2015ALBIace15KG |
| SEA Lysate Titer | 1.1 x 10^7 |
| Date of SEA Lysate Titering | Nov 23, 2015 |
| Pitt Freezer Box# | 22 |
| Pitt Freezer Box Grid# | A1 |