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Mycobacterium phage ACE15
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Detailed Information for Phage ACE15
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByK. Gilbert, M. Osterndorf, M. Bertels, B.Flanagon
Year Found2015
Location FoundAlbion, MI USA
Finding InstitutionAlbion College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates84.742457 N, 42.240537 E Map
Discovery NotesA soil sample was obtained from the grounds of Albion College, and a suspension of it was centrifuged in order to separate the components by density. The liquid supernatant was extracted from the centrifuged material. We used serial dilutions of supernatant to start growing phages in smeg cultures, using both further series of dilutions and streaking to isolate a single strain of phage, which was extracted into a medium titer lysate. Once this was completed, we did a gel electrophoresis and used that sample to extract DNA and obtained an electron micrograph of individual virions.
Naming NotesAlbion College Equestrian year 2015
Sequencing Information
Sequencing Complete?No
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2015ALBIace15KG
SEA Lysate Titer 1.1 x 10^7
Date of SEA Lysate Titering Nov 23, 2015
Pitt Freezer Box# 22
Pitt Freezer Box Grid# A1