Mycobacterium phage Adam007
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Detailed Information for Phage Adam007 | |
Discovery Information | |
Isolation Host | Mycobacterium smegmatis mc²155 |
Found By | Azqa Qureshi |
Year Found | 2017 |
Location Found | Dahlonega, GA USA |
Finding Institution | University of North Georgia |
Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
From enriched soil sample? | Yes |
Isolation Temperature | Not entered |
GPS Coordinates | 34.5291 N, 83.9881 W Map |
Discovery Notes | The soil where I got the sample was covered in wood chips, so I had to dig a little ways down to reach the moist soil. I got a few small twigs and little pebbles in the sample itself. The sample was granular and dark brown in color. |
Naming Notes | At first, I wanted to name my phage “Adam”, like Adam from the Bible, since it was “the first phage” I found. This name was already taken, though, so I put “007” at the end of it because I am a huge James Bond fan. |
Sequencing Information | |
Sequencing Complete? | No |
Genome length (bp) | Unknown |
Character of genome ends | Unknown |
Fasta file available? | No |
Characterization | |
Cluster | Unclustered |
Subcluster | -- |
Annotating Institution | Unknown or unassigned |
Annotation Status | Not sequenced |
Plaque Notes | The plaque from my phage are fairly uniform in size; however, there are a few that were larger than others. This is a tell-tale sign that I have different kinds of bacteriophage on my plate. My average plaque size is 0.5 mm. My phage has a high adsorption rate, since I have many plaques on each plate. Although the incubation rate affects the size of the plaque significantly, there are some outside variables like agar that I personally have very little control over. This could affect the number of plaques. For example, the moisture on the agar plates may have decreased the number of plaques I could have obtained had the collected water droplets not have coated the agar/bacteria/phage sample/7H9. The plaques are very clear and there is almost no turbidity. |
Morphotype | Siphoviridae |
Has been Phamerated? | No |
Publication Info | |
Uploaded to GenBank? | No |
GenBank Accession | None yet |
Refseq Number | None yet |
Archiving Info | |
Archiving status | Not in Pitt Archives |
SEA Lysate Titer | 5x10e9 |
Available Files | |
Plaque Picture | Download |
EM Picture | Download |