Mycobacterium phage Adam007
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| Detailed Information for Phage Adam007 | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Azqa Qureshi |
| Year Found | 2017 |
| Location Found | Dahlonega, GA USA |
| Finding Institution | University of North Georgia |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 34.5291 N, 83.9881 W Map |
| Discovery Notes | The soil where I got the sample was covered in wood chips, so I had to dig a little ways down to reach the moist soil. I got a few small twigs and little pebbles in the sample itself. The sample was granular and dark brown in color. |
| Naming Notes | At first, I wanted to name my phage “Adam”, like Adam from the Bible, since it was “the first phage” I found. This name was already taken, though, so I put “007” at the end of it because I am a huge James Bond fan. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaque from my phage are fairly uniform in size; however, there are a few that were larger than others. This is a tell-tale sign that I have different kinds of bacteriophage on my plate. My average plaque size is 0.5 mm. My phage has a high adsorption rate, since I have many plaques on each plate. Although the incubation rate affects the size of the plaque significantly, there are some outside variables like agar that I personally have very little control over. This could affect the number of plaques. For example, the moisture on the agar plates may have decreased the number of plaques I could have obtained had the collected water droplets not have coated the agar/bacteria/phage sample/7H9. The plaques are very clear and there is almost no turbidity. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 5x10e9 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |