Streptomyces phage Aginita
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| Detailed Information for Phage Aginita | |
| Discovery Information | |
| Isolation Host | Streptomyces mirabilis NRRL B-2400 |
| Found By | Amarachukwu Izugha and Alexis Gantt |
| Year Found | 2025 |
| Location Found | Catonsville, MD United States of America |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.256199 N, 76.712371 W Map |
| Discovery Notes | The soil sample was taken from a pond located on campus after a rainy day. The soil sample was wet when collected that night. |
| Naming Notes | Decided on combining two words from each language we are actively learning to create a name. 'Agi' means 'baby' in Korean, and 'niñita' means 'little girl' in Spanish. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Lysogeny Notes | When the bacteria isolated from the plaque mesas were tested, no new plaque formation or lytic activity occurred, indicating that Aginita is a highly lytic phage rather than a temperate one. This suggests that Aginita does not establish lysogeny within its host but instead causes complete lysis upon infection. |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The plaques observed during purification were approximately 0.30 cm in diameter. They appeared round and uniform in shape, with a distinct small halo surrounding each plaque. The presence of the halo suggests possible diffusion of phage-encoded enzymes into the surrounding bacterial lawn, which may indicate enzymatic activity or partial clearing beyond the central lytic zone. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 4.2 * 10^7 PFU/mL |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
