Mycobacterium phage Ambition
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Detailed Information for Phage Ambition
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMeredith Doughty
Year Found2011
Location FoundBowling Green, KY USA
Finding InstitutionWestern Kentucky University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates36.946305 N, 86.408583 W Map
Discovery NotesFound by the house gutter. September 4, 2011 at 9:18am
Elevation: 540.1 ft
Weather: 78° F
Barometer: 29.9 in
Dewpoint 69° F
Humidity: 74%
Precip within the last 24 hours: 0.41in
Wind: 3mph SW
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesVery turbid (plaques are not clear), which probably means that the phage is lysogenic. It also has a "halo" around a dark center. The plaque entirely is a bit smaller than D29 plaques, which could mean it has more phage than smaller plaques would.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Designator 2011WEKUAmbitionMAD
SEA Lysate Titer 1.62e8
Date of SEA Lysate Titering Oct 4, 2011
Pitt Freezer Box# 31
Pitt Freezer Box Grid# 66
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload