Arthrobacter phage Angstig
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| Detailed Information for Phage Angstig | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Rukmini Ganesh |
| Year Found | 2016 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 36.978889 N, 86.466667 W Map |
| Discovery Notes | 510 ft elevation 82 degrees Fahrenheit Sunny Collected 9/19/2016 at 6:09 pm Incubation temperature: 30 C Calcium concentration: 2 milliMolar sample was enriched. Phage adopted from Mason Tomko. Soil collected from a Waffle House near a roadway and railroad track, by theh trash containment. Litter, weeds, and dead grass were scattered through the site. Waffle House address: 1868 Russellville Rd, Bowling Green, KY 42102 Phages Djoodi (Tomko) and BirdPerson (Simpson) were isolated from the same enriched soil sample. |
| Naming Notes | The phage name is Dutch for anxiety, the main emotion felt during its discovery, isolation, and purification. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | After 48 hours of incubation at 30C, plaques are typically approximately 1.5 mm in diameter and slightly unclear edges. The plaques are clear, not turbid, indicating a lytic phage. The phage requires a concentration of 2mM calcium, and no antibiotics. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | 2016WEKUangstigRG |
| SEA Lysate Titer | 8.3x10^9 pfu/mL |
| Date of SEA Lysate Titering | Oct 25, 2016 |
| Pitt Freezer Box# | 37 |
| Pitt Freezer Box Grid# | B1 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
