Mycobacterium phage AuntSally
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Detailed Information for Phage AuntSally
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByMel Buzzanga
Year Found2022
Location FoundManchester, NH United States
Finding InstitutionUniversity of New Hampshire at Manchester
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature16°C
GPS Coordinates42.994319 N, 71.467308 E Map
Discovery NotesThe soil was full of sticks and pebbles. The sample in question was collected from under some leaves at the base of a tree across the street from the UNH Manchester campus.
Naming NotesIn one of the early days of my class' phage discovery journey, we were working on our first serial dilution, which includes doing a "Meet and Greet" with host bacteria. I was having trouble getting the bacteria to move up the tube and hit the serial dilution sample on the wall of said tube and said "Come on, go hug your dear Aunt Sally, she misses you." After asking what in the world I was talking about, one of my labmates said that I had to name my phage "Aunt Sally" if what I was plating that day turned out to be a viable phage. It was not, but I liked the name enough to use it for my next samples, and here we are. Funnily enough, I actually don't have an Aunt Sally.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesIt appears to be cloudy and lytic, with no visible margin. The plaques are approximately 1mm in diameter.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 153
Pitt Freezer Box Grid# D7
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload