Mycobacterium phage B2Spirit
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| Detailed Information for Phage B2Spirit | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Nathan Brodjeski, Trieu Lam |
| Year Found | 2024 |
| Location Found | Boulder, CO United States |
| Finding Institution | University of Colorado Boulder |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 32°C |
| GPS Coordinates | 39.6464 N, 104.726 W Map |
| Discovery Notes | Sample was collected in area where onions were grow. It was collected on 09/03/2024 at 12:25pm. |
| Naming Notes | Order for the Digest Picture. Restriction digest shows EcoRI enzyme cut down our phage DNA. From left to right: Control, BamHI – 2uL, ClaI – 2uL, EcoRI -2uL, HaeIII -2 uL, HindIII -2uL, SalI – 2uL, DNA Ladder. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The image reveals the presence of both lytic and lysogenic phages: the lytic phages, indicated in red, appear as small clear circles, while the lysogenic phages, shown in blue, shown as larger, cloudy circles. Sample displays a predominance of lysogenic phages, characterized by big cloudy spots. Additionally, the distribution of phages in the sample was not uniform, which may have resulted from an inadequate technique in spreading the solution onto the agar disk. |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |