The Actinobacteriophage Database

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Detailed Information for Phage Bernal13
Discovery Information
Isolation Host	Mycobacterium smegmatis mc²155
Found By	Daniel Bernal
Year Found	2012
Location Found	Gainesville, FL USA
Finding Institution	University of Florida
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	Not entered
GPS Coordinates	29.625 N, 82.366 W Map
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 29, 2013
Sequencing Facility	Virginia Commonwealth University Nucleic Acids Research Facilities
Shotgun Sequencing Method	454 Pyrosequencing
Approximate Shotgun Coverage	270
Sanger Finishing Reactions	8
Genome length (bp)	42392
Character of genome ends	3' Sticky Overhang
Overhang Length	11 bases
Overhang Sequence	CGAAACGCCAT
GC Content	66.2%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	T
Subcluster	--
Cluster Life Cycle	Temperate
Other Cluster Members	Click to View Bernal13 Ibrahim Mendokysei Nairb RonRayGun Whitty ZenTime222
Annotating Institution	Unknown or unassigned
Annotation Status	In GenBank
Plaque Notes	Very small plaques about 1 mm in diameter. Opaque plaques.
Morphotype	Siphoviridae
Number of Genes	60
Number of tRNAs	0
Number of tmRNAs	0
Has been Phamerated?	Yes
Gene List	Click to View Bernal13_1 Bernal13_2 (terminase small subunit) Bernal13_3 (terminase) Bernal13_4 (portal protein) Bernal13_5 (MuF-like minor capsid protein) Bernal13_6 Bernal13_7 (scaffolding protein) Bernal13_8 (major capsid protein) Bernal13_9 Bernal13_10 Bernal13_11 Bernal13_12 Bernal13_13 Bernal13_14 (major tail protein) Bernal13_15 (tail assembly chaperone) Bernal13_16 (tail assembly chaperone) Bernal13_17 (tape measure protein) Bernal13_18 (minor tail protein) Bernal13_19 (minor tail protein) Bernal13_20 (minor tail protein) Bernal13_21 (minor tail protein) Bernal13_22 (minor tail protein) Bernal13_23 Bernal13_24 (minor tail protein) Bernal13_25 (minor tail protein) Bernal13_26 Bernal13_27 (lysin A) Bernal13_28 (holin) Bernal13_29 Bernal13_30 Bernal13_31 Bernal13_32 Bernal13_33 (tyrosine integrase) Bernal13_35 (immunity repressor) Bernal13_36 (helix-turn-helix DNA binding protein) Bernal13_37 (excise) Bernal13_38 Bernal13_39 Bernal13_40 Bernal13_41 (WhiB family transcription factor) Bernal13_42 Bernal13_43 Bernal13_44 (RecB-like exonuclease/helicase) Bernal13_45 Bernal13_46 Bernal13_47 Bernal13_48 Bernal13_49 (helix-turn-helix DNA binding protein) Bernal13_50 Bernal13_51 Bernal13_52 Bernal13_53 Bernal13_54 Bernal13_55 Bernal13_56 Bernal13_57 Bernal13_58 Bernal13_59 (HNH endonuclease) Bernal13_60 (DNA methylase) Bernal13_61
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	KJ510413
Refseq Number	NC_024135
Published in Paper	Pope, Bowman, Russell, et al 2015, eLIFE: Whole genome comparison of a large collection of mycobacteriophages reveals a continuum of phage genetic diversity
Archiving Info
Archiving status	Archived
SEA Lysate Titer	2.1 x10^11
Pitt Freezer Box#	40
Pitt Freezer Box Grid#	60
Available Files
Restriction Digest Picture	Download
Virtual Digest Picture	Download
EM Picture	Download

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