Mycobacterium phage Biddle
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| Detailed Information for Phage Biddle | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Former names | Egahpb |
| Found By | Matthew Biddle |
| Year Found | 2015 |
| Location Found | Bowling Green, KY USA |
| Finding Institution | Western Kentucky University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 37°C |
| GPS Coordinates | Unavailable |
| Discovery Notes | I collected this sample at the edge of a field by a brick column at the edge of two concrete walkways. This spot gets shade for half the day and is subject to sunlight the other half. I collected my sample at 2:15 on a sunny afternoon, it had not rained recently and the soil was rather dry. It did not appear to have any evidence of heavy traffic. This was also on a college campus. The soil appeared to be of a red clay variety and was collected at a depth of 7 cm below the surface. |
| Naming Notes | I named my bacteriophage after myself, Biddle. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | My plaques are clear yet exhibit small amounts of turbidity making them slightly opaque but still easily seen through. My average plaque size is near 3 mm after 24 hours. The plaques exhibit a growth in size between 24-48 hours and when grown at 37 degrees and 30 degrees appeared to not develop at differing rates. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 3.4x10^11 |
| Date of SEA Lysate Titering | Nov 5, 2015 |
| Pitt Freezer Box# | 27 |
| Pitt Freezer Box Grid# | D11 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
