Mycobacterium phage Bohemian
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| Detailed Information for Phage Bohemian | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Corinne Chaplin & Christopher Dolce |
| Year Found | 2017 |
| Location Found | Philadelphia, PA USA |
| Finding Institution | University of the Sciences (now St. Joseph's Univ) |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.9468 N, 75.2071 E Map |
| Discovery Notes | The soil sample was taken from a garden. While purifying our phage, we found that it had a very low titer (1.01x10^8 for low volume lysate, 3.22x10^8 for high volume lysate). |
| Naming Notes | We named our phage Bohemian because when we sent it off to be imaged under the Electron Microscope, the technician was only able to find a single phage on our grid, and almost gave up on finding it! However, they found our lone ranger, and the images of it came back very clear. It wanders around, killing mycobacterium smegmatis; a true bohemian. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | Clear in center, turbid at edges, ~1/10 cm in diameter. |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 47 |
| Pitt Freezer Box Grid# | B6 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
