Mycobacterium phage Bohemian
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Detailed Information for Phage Bohemian
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByCorinne Chaplin & Christopher Dolce
Year Found2017
Location FoundPhiladelphia, PA USA
Finding InstitutionUniversity of the Sciences in Philadelphia
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates39.9468 N, 75.2071 E Map
Discovery NotesThe soil sample was taken from a garden. While purifying our phage, we found that it had a very low titer (1.01x10^8 for low volume lysate, 3.22x10^8 for high volume lysate).
Naming NotesWe named our phage Bohemian because when we sent it off to be imaged under the Electron Microscope, the technician was only able to find a single phage on our grid, and almost gave up on finding it! However, they found our lone ranger, and the images of it came back very clear. It wanders around, killing mycobacterium smegmatis; a true bohemian.
Sequencing Information
Sequencing Complete?No
Genome length (bp)Unknown
Character of genome endsUnknown
Fasta file available?No
Characterization
ClusterUnclustered
Subcluster--
Annotating InstitutionUnknown or unassigned
Annotation StatusNot sequenced
Plaque NotesClear in center, turbid at edges, ~1/10 cm in diameter.
MorphotypeSiphoviridae
Has been Phamerated?No
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 47
Pitt Freezer Box Grid# B6
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload