Mycobacterium phage Bolts
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| Detailed Information for Phage Bolts | |
| Discovery Information | |
| Isolation Host | Mycobacterium smegmatis mc²155 |
| Found By | Danielle Obando |
| Year Found | 2015 |
| Location Found | Baltimore, MD USA |
| Finding Institution | Johns Hopkins University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | Unavailable |
| Discovery Notes | Soil sample collected August 27, 2015 around 9 AM, at Wyman Park. Sample was collected from underneath a bridge where the soil was not directly hit by the sun. The soil was clay-like. |
| Naming Notes | The name was inspired by various qualities of the phage including its robustness indicated by its stability at room temperature. Also, the rate of bacterial lysis made it difficult to culture 6 web plates; although, appropriate calculations were done, the entire plate would result clear rather than webbed. I felt that the various meanings of the term 'bolt' accurately encompassed the "stubborn" and "speedy" nature of this phage. |
| Sequencing Information | |
| Sequencing Complete? | No |
| Genome length (bp) | Unknown |
| Character of genome ends | Unknown |
| Fasta file available? | No |
| Characterization | |
| Cluster | Unclustered |
| Subcluster | -- |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | Not sequenced |
| Plaque Notes | The clear nature of the phage plaque indicates that the phage is in the lytic cycle. Plaque sizes tend to differ, ranging from smaller plaques to larger plaques. Additional streaking of plaques of various sizes was done in order to assure an isolated phage was obtained. The head to tail length was nearly one-to-one (tail length: 199.58; head length: 207.18). Higher-Titer Lysate is stable at room temperature. |
| Morphotype | Myoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 5.4e9 |
| Date of SEA Lysate Titering | Oct 27, 2015 |
| Pitt Freezer Box# | 71 |
| Pitt Freezer Box Grid# | C8 |