| Detailed Information for Phage Bran | |
| Discovery Information | |
| Isolation Host | Corynebacterium xerosis ATCC 373 |
| Found By | Kelly Kim |
| Year Found | 2018 |
| Location Found | Birmingham, AL USA |
| Finding Institution | University of Alabama at Birmingham |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 33.543682 N, 86.779633 W Map |
| Discovery Notes | Bran was isolated from an enriched sewage sample. |
| Naming Notes | Not a huge fan of Raisin Bran but always admired the name "Bran" |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 3, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1106 |
| Genome length (bp) | 44484 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 17 bases |
| Overhang Sequence | CCCCAGCGCCTCGGGTA |
| GC Content | 67.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | EP |
| Subcluster | -- |
| Cluster Life Cycle | Unknown |
| Lysogeny Notes | Signs of potential lysogen formation appeared when distinct halos formed during the spot titer assay, which was executed to find the titer of the medium titer lysate. After successfully testing that the phage cannot infect itself, it is highly possible for Bran to form stable lysogens. |
| Other Cluster Members | |
| Annotating Institution | University of Alabama at Birmingham |
| Annotation Status | In GenBank |
| Plaque Notes | After performing 4 rounds of phage titer assay to isolate one type of phage, 103 small and separate plaques formed on the 10^8 dilution plate after incubating in 37°C after 48 hours. A high titer lysate (HVL) was acquired utilizing this dilution, and the titer came out to be 2.06x10^11 pfu/mL. |
| Number of Genes | 69 |
| Number of tRNAs | 1 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewBran_1 Bran_2 Bran_3 Bran_4 Bran_5 Bran_6 Bran_7 Bran_8 Bran_9 Bran_10 Bran_11 Bran_12 Bran_13 Bran_14 Bran_15 Bran_16 Bran_17 Bran_18 Bran_19 Bran_20 Bran_21 Bran_22 Bran_23 Bran_24 Bran_25 Bran_26 Bran_27 Bran_28 Bran_29 Bran_30 Bran_31 Bran_32 Bran_33 Bran_34 Bran_35 Bran_36 Bran_37 Bran_38 Bran_39 Bran_40 Bran_41 Bran_42 Bran_43 Bran_44 Bran_45 Bran_46 Bran_47 Bran_48 Bran_49 Bran_50 Bran_51 Bran_52 Bran_53 Bran_54 Bran_55 Bran_56 Bran_57 Bran_58 Bran_59 Bran_60 Bran_61 Bran_62 Bran_63 Bran_64 Bran_65 Bran_66 Bran_67 Bran_68 Bran_69 — tRNA Bran_70 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK977714 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 89 |
| Pitt Freezer Box Grid# | C1 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |